Clone ID: E17
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1017-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP, FC
Species Reactivity: Human, Mouse
Mol. Weight: 26 kDa
UniProt ID: P10415
Alternative names:
Description: The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4). Specificity: A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 was used as immunogen. The antibody does not cross-react with other Bcl-2 family members
Recommended Dilutions:
WB: 1:500
IHC: 1:100-1:200
ICC: 1:00
Flow Cytometry: 1:200
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western
blot analysis of Jurkat cell lysate using anti-Bcl-2 RabMab
(Cat#1017-1), 1:1,000 dilution.
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B. Immunohistochemical
analysis of paraffin-embedded human breast carcinoma using
anti-BCL-2 Rab Mab (Cat#1017-1). |
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C. Flow cytometric analysis of permeabilized Jurkat cells using anti-Bcl-2 RabMAb (red)(catalog #1017-1)or a rabbit IgG (negative) (green). |
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Product References:
| 1. | Deng J, Carlson N, Takeyama K, et al, BH3 Profiling Identifies Three Distinct Classes of ApoptoticBlockstoPredictResponsetoABT-737 and Conventional Chemotherapeutic Agents ,Cancer Cell 12, 171?185, August 2007
[Application: WB, IP] | | 2. | Eliseev RA et. al., Increased radiation-induced apoptosis of Saos2 cells via inhibition of NFB: A role for c-Jun N-terminal kinase ,Journal of Cellular Biochemistry 96:1262-1273 (2005).
[Application: WB] |
Description References:
| 1. |
Hockenbery, D. et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334 |
| 2. |
Cleary, M.L. et al. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell 47: 19 |
| 3. |
Ruvolo, P.P et al. Phosphorylation of Bcl2 and regulation of apoptosis. Leukemia 15: 515 |
| 4. |
Deng, X. et al. Novel Role for JNK as a Stress-activated Bcl2 Kinase. J. Biol. Chem. 276: 23681 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
