Clone ID: Y8
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1008-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP, FC
Species Reactivity: Human
Mol. Weight: 22 kDa
UniProt ID: P55957
Alternative names: BID, p22 BID
Description: Bid, BH3 interacting domain death agonist, is a pro-apoptotic member of the Bcl-2 family of proteins that regulate apotosis. Bid is localized to the cytosol in an inactive precursor form. Upon caspase-8 binding in the Fas signaling pathway, Bid is cleaved and the active Bid fragment translocates to mitochondria and induces cytochrome c release and the resulting apoptotic cascade (1-3). Bid contains a BH3 domain which allows it to dimerize with and counter the death repressor effects of Bcl-2. BID has also been shown to heterodimerize with Bcl-x and the death agonist Bax (4). Specificity: A synthetic peptide corresponding to residues before the BH3 domain of human Bid was used as the immunogen. This antibody does not cross-react with other Bcl-2 family members.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:100
ICC: 1:100
Flow Cytometry: 1:50
IP: 1:10
Protocols for using Epitomics Rabbit Monoclonal Antibodies
 |
 |
| A. Western
blot analysis of Jurkat cell lysate using anti Bid RabMAb catalog#
1008 -1 |
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-Bid RabMAb (catalog # 1008-1). |
Description References:
| 1. |
Gross, A. et al. Caspase Cleaved BID Targets Mitochondria and Is Required for Cytochrome c Release, while BCL-XL Prevents This Release but Not Tumor Necrosis Factor-R1/Fas Death. J. Biol. Chem. 274, 1156 |
| 2. |
Li, H. et al. Cleavage of BID by Caspase 8 Mediates the Mitochondrial Damage in the Fas Pathway of Apoptosis. Cell 94, 491 |
| 3. |
Luo, X. et al. Bid, a Bcl2 Interacting Protein, Mediates Cytochrome c Release from Mitochondria in Response to Activation of Cell Surface Death Receptors. Cell 94, 481 |
| 4. |
Wang, K. et al. BID: a novel BH3 domain-only death agonist. Genes Dev. 10(22): 2859 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
