Why RabMAbs® make better Akt antibodies:
Akt1/PKBα is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including
apoptosis, glycogen synthesis and cell growth. Akt1/PKBα is activated by various growth and survival factors, where
phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBα inhibits apoptosis by
phosphorylating and inactivating several targets, including caspase-9.
At Epitomics, we provide a wide range of high quality antibodies to Akt and related proteins. Using our patented
Rabbit Monoclonal Antibody (RabMAb®) technology, we have developed a collection of high quality Akt specific
antibodies. The rabbit immune system has a much better response to small epitopes than the mouse immune system, allowing the development of high quality antibodies to subtle changes in epitopes such as post-translational modifications and activations (1,2). Therefore, each Akt specific RabMAb offers the benefits of superior antigen recognition of the rabbit immune system and the
specificity of a monoclonal antibody.
Epitomics validates every RabMAb in western blot, immunohistochemistry, immunofluorescence, immunoprecipitation and flow cytometry before release, so you can be assured of which assays the antibody will work in.
Complete list of Akt Antibodies >>
More about the advantanges of Rabbit Monoclonal Antibodies >>
Featured Akt RabMAbs
Akt Phospho (pT308) |
Akt Phospho (pS473) |
Akt Phospho (pT450) |
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| Western blot analysis on NIH/3T3 cell lysates using anti-Phospho-Akt1 (pT308) RabMAb (cat. #2214-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with PDGF. | Immunohistochemical analysis of Phosphatase-untreated (left) and treated (right) cervical carcinoma using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1). | Western blot analysis on NIH/3T3 cell lysate using anti-Akt1 Phospho (pT450) RabMAb (cat. #3188-1). Cells were either (A) untreated or (B) treated with Lambda Phosphatase. |
Catalog #2214-1
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Catalog #2118-1
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Catalog #3188-1
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References
1.
A humanized anti-VEGF rabbit monoclonal antibody inhibits angiogenesis and blocks tumor growth in xenograft models Yu Y, et al., [PLoS One. 2010 Feb 5;5(2):e9072]
2. Rabbit monoclonal antibody: potential application in cancer therapy Lifeng Feng, Xian Wang, and Hongchuan Jin [Am J Transl Res. 2011 May 15; 3(3): 269–274]
