EpiMAX™ Affinity Purification Kit
EpiMAX™ Affinity Purification kit is a complete antibody purification solution for quick and easy purification of ultra pure antibodies. The EpiMAX™ Affinity Purification kit is compatible with manual or automated procedures and small or large scale antibody purifications.
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| Fig 1. Simple Reaction Scheme for coupling of peptide/protein to ASA gel matrix |
EpiMAX™ Affinity Purification kit is based on Epitomics ASA (Activated Serial-A) Matrix Gel Technology which provides multiple benefits –
- Easy column preparation with highly stable covalent immobilization of any SH- or NH3- containing target peptide/protein (Figure 1)
- Immobilize both peptide and protein of any size and at various buffer and temperature conditions (Table 1).
- Superior peptide coupling efficiency and binding capacity compared to traditional peptide based antibody purification (Figure 2)
EpiMAX™ Affinity Purification kit requires less purification time and provides higher yield of ultra pure (>95% purity) antibody compared to traditional peptide purification methods (Figures 3-5).
For Automated purification, EpiMAX™ Affinity Purification kit includes adjustable column adaptor for easy conversion between gravity and pump based purification Click Here Details
Click to Enlarge
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Fig 2. A comparsion of minimum and maximum values of peptide binding coupling efficiency (CE%) and antibody binding capacity (mg/ml of gel) between EpiMAX™ and traditional peptide based purification kit.
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Fig 3. A comparsion of total purification steps and time between EpiMAX™ and traditional peptide based purification kit
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Fig 4. A comparsion of minimum and maximumtarget antibody recovery (%) between EpiMAX™ and traditional peptide based purification kit |
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Condition |
Compatible Range |
Temperature |
4°C to 50°C |
pH |
6 to 10 |
Solvent Type |
Organic and Inorganic |
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| Table 1. Compatible peptide/protein coupling conditions for ASA coupling gel matrix included in EpiMAX™ Affinity Purification Kit. |
Fig 5. Western blot analysis on Jurkat cell lysate using Cyclin B1 RabMAb A) Crude antisera, B) flow-thru, C) EpiMAX™ Purified. Target band at 58 kDa. |
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Product Listing
Product Name |
Type |
Cat# |
Price |
Buy Now  |
EpiMAX™ Affinity Purification Kit |
Kit (purification up to 5 different antibodies) |
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$395 |
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EpiMAX™ Affinity Purification Kit Manual
Requirements :
• Peptide for conjugation to the column must have SH- or NH3- terminal group.
• Sample requirement is as follows: ELISA based sample reading at 1:16K Dilution factor
  should be equal or greater than 0.50 (OD 280 reading).
List of Components :
•Activated Coupling Gel*
•Antigen Coupling Buffer
•Reductant Sodium
•Reductant Solution
•Peptide Dissolving Buffer
•Antisera Sample Buffer
•Antibody Elution Buffer
•Antibody Neutralization Buffer
•Chromatography Columns (5)
•Column Adaptor for Pump Usage (1)
*Bulk Size Available - contact info@epitomics.com or call 877-772-2622
SPECIAL OFFER - Order 3 purification kits and receive 40% Discount
on the EpiMAX automated pump!
- Click Here for More Info and Other Available Promotions -
EpiMAX™ Automated Pump
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For automated pump purification, EpiMAX™ Affinity Purification kit is optimized to be used with Epitomics EpiMAX™ automated pump (Cat# APS-D100NA, APS-D100EA ).
EpiMAX™ Automated Pump User Manual
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Epitomics EpiMAX™ Automated Pump - APS-D100NA, APS-D100EA |
Product Listing
Product Name |
Type |
Cat# |
Price |
Buy Now  |
EpiMAX™ Automated Pump - North America Version |
Syringe Pump (120 volts) |
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$895 |
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EpiMAX™ Automated Pump - Asia/Europe Version |
Syringe Pump (220 volts) |
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Please Inquire |
Pump Specifications:
| Syringe Adaptability |
Standard 20, 30, 50 ml Syringes |
| Volume Limit |
0-9999 ml |
| Accuracy |
≤±2% |
| Syringe Volume |
20ml |
30ml |
50ml |
| Flow Rate |
0.1-399.9ml/h |
0.1-600.0ml/h
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0.1-1200.0ml/h |
| Rapid Rate |
399ml/h |
600.0ml/h |
1200.0ml/h |
| Dimensions |
307mm (L) x 135mm (W) x 128 mm (H) |
| Net Weight |
2.1 kg |
| Operation Conditions |
5 - 40°C, RH: 20 - 40 % |
| Storage Conditions |
-40 - 55°C, RH: 95% |
View the complete list of Epitomics RabMAbs.
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