Epitomics PEG ELISA Kit

Polyethylene glycol (PEG) is an O-CH2-CH2 polymer, which is water-soluble, nontoxic, nonantigenic, and biocompatible. Covalent conjugation of PEG to therapeutic proteins increases their in vivo stability by protecting the protein from degradation, masking its immunogenic sites and reducing clearance (1). Typically, PEGylation uses nonspecific reactions with nucleophilic residues and produces mixtures of PEGylated positional isomers (2). Qualitative and quantitative analysis of PEGylated molecules is important for both drug development and clinical applications. Therefore, this kit is developed to determine levels of PEGylated molecules in samples such as serum via ELISA.

Fig 1. Competition between PEG labeled HRP (H) and PEG labeled sample (S) in PEG ELISA assay.

The PEG ELISA kit operates on the basis of competition between the enzyme (HRP) conjugated PEG and PEG labeled molecules for a limited number of binding sites on the surface of 96-wells coated with anti-PEG antibody. The extent of color development resulted from interaction between HRP and substrate TMB is inversely proportional to the amount of PEGylated molecules in the sample (Figure 1.).

Example Data

List of Components :
• Dry Plate with stripwells (96)
• PEG-BSA Reference Standard
• PEG conjugated HRP
• Antigen/Antibody Diluent Buffer
• ELISA Washing Buffer
• TMB A Substrate Solution
• TMB B Substrate Solution
• Stop Solution

*Bulk Quantities Available - contact info@epitomics.com or call 877-772-2622

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